The Cannabis – Terpene Synergy

by in Blog · Full · Medical · Science — 6 Mar, 2015

terpenes

When selecting among varieties of dried cannabis at a dispensary, members will often ask to smell the particular strain for it’s signature scent. The musky smell commonly associated with the Kush family comes from an abundance of a terpene called Myrcene, known for it’s sedative effects, also found in hops (Humulus), the only other member of the Cannabaceae plant family. The Piney smell is Alpha-pinene (essential pine oil), known to promote alertness and memory retention. Lemoney sativa strains contain limonene, which anecdotal evidence suggests is “sunshine-y,” and is also found in, you guessed it, lemons.

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(fig. 1, trichome cross section)

Terpenes are aromatic compounds that are produced alongside cannabinoids in the rosette of cells that holds up the head of the trichome (fig. 1). Most of the terpenes that create the many scents of cannabis are shared among the plant kingdom. The Aug. 2011 British Journal of Pharmacology: Cannabinoids in Biology and Medicine, Part 1 includes numerous articles exploring the nature of the cannabis plants’ chemical dynamism. In the article “Taming THC,” scientists explored how these aromatic oils synergize and mitigate the active cannabinoids contributing to an entourage effect.

Traditional responses to cannabis induced anxiety include sniffing pinene-rich black pepper, limonene-rich citrus, and calamus root high in myrcene. “Cannabis terpenoids and flavonoids may also increase cerebral blood flow, enhance cortical activity, kill respiratory pathogens, and provide anti-inflammatory activity.” (source) Ed Rosenthal, author of many books on cannabis, relates that the myrcene in mangos can increase the quality of low potency cannabis when eaten one hour before medicating. A study launched by David Watson and Robert Clarke for Holland based research company Hortipharm found that terpene-infused resin with 50 percent THC was more potent by dry weight than an equivalent amount of pure THC.

Scientists have discovered that beta-caryophyllene (BCP), which is another terpene that contributes to the aroma and flavour, also found in other herbs, spices, and food plants, activates the CB2 receptor and acts as a non-psychoactive anti-inflammatory. Because it binds to a cannabinoid receptor […] and since it is an FDA approved food additive and ingested daily with food, it is the first known dietary cannabinoid. (source)

Terpenes break down over time, if you can smell it, you’re losing it. Some terpenes are volatile below room temperature: smaller, lighter terpenes (monoterpenes) like myrcene and limonene evaporate faster. Bigger, heavier terpenes (sesquiterpenes) like caryophyllene don’t evaporate as quickly, and represent a larger percentage of the oil after drying. (Study) Careful handling and storage can help to prevent the breakdown of the cannabinoids and terpenes, choose a cool, dry, dark place (out of the reach of children and pets) to store your cannabis in a sealed container.

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Leading the way on terpene identification is Green House Seed Company in Holland who have performed spectral analysis of each of their strains identifying 16 different terpenes. They have developed an odor wheel to help individuals decide on their strain of choice. LiftCannabis.com offers a system by which you can select your strains by the flavours.

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CO2-Enriched Air Improves Plant Medicinal Properties

—  Reprinted from the following Link:    http://www.plantsneedco2.org/default.aspx?menuitemid=398

Primitive medical records indicate that extracts from many species of plants have been used for treating a variety of human health problems for perhaps the past 3500 years (Machlin, 1992; Pettit et al., 1993, 1995).  In modern times the practice has continued, with numerous chemotherapeutic agents being isolated (Gabrielsen et al., 1992a).  Until recently, however, no studies had investigated the effects of atmospheric CO2 enrichment on specific plant compounds of direct medicinal value.

This situation changed when Stuhlfauth et al. (1987) studied the individual and combined effects of atmospheric CO2 enrichment and water stress on the production of secondary metabolites in the woolly foxglove (Digitalis lanata EHRH), which produces the cardiac glycoside digoxin that is used in the treatment of cardiac insufficiency.  Under controlled well-watered conditions, a near-tripling of the air’s CO2 content increased plant dry weight production in this medicinal plant by 63%, while under water-stressed conditions the CO2-induced dry weight increase was 83%.  In addition, the concentration of digoxin within the plant dry mass was enhanced by 11% under well-watered conditions and by 14% under conditions of water stress.

In a subsequent whole-season field experiment, Stuhlfauth and Fock (1990) obtained similar results.  A near-tripling of the air’s CO2 concentration led to a 75% increase in plant dry weight production per unit land area and a 15% increase in digoxin yield per unit dry weight of plant, which combined to produce an actual doubling of total digoxin yield per hectare of cultivated land.

In another study, Idso et al. (2000) evaluated the response of the tropical spider lily (Hymenocallis littoralis Jacq. Salisb.) to elevated levels of atmospheric CO2 over four growing seasons.  This plant has been known since ancient times to possess anti-tumor activity; and in modern times it has been shown to contain constituents that are effective against lymphocytic leukemia and ovary sarcoma (Pettit et al., 1986).  These same plant constituents have also been proven to be effective against the U.S. National Cancer Institute’s panel of 60 human cancer cell lines, demonstrating greatest effectiveness against melanoma, brain, colon, lung and renal cancers (Pettit et al., 1993).  In addition, it exhibits strong anti-viral activity against Japanese encephalitis and yellow, dengue, Punta Tora and Rift Valley fevers (Gabrielsen et al., 1992a,b).

The results of the Idso et al. study showed that a 75% increase in the air’s CO2 concentration produced a 56% increase in the spider lily’s belowground bulb biomass, where the disease-fighting substances are found.  In addition, for these specific substances, they observed a 6% increase in the concentration of a two-constituent (1:1) mixture of 7-deoxynarciclasine and 7-deoxy-trans-dihydronarciclasine, an 8% increase in pancratistatin, an 8% increase in trans-dihydronarciclasine, and a 28% increase in narciclasine.  Averaged together and combined with the 56% increase in bulb biomass, these percentage concentration increases resulted in a total mean active-ingredient increase of 75% for the plants grown in air containing 75% more CO2.

In another study, Zobayed and Saxena (2004) worked with St. Johns’ wort, a perennial herb native to Europe and West Asia that has been used as a medicinal plant for the treatment of mild to moderate depression, inflammation and wound healing (Brolis et al., 1998; Stevinson and Ernst, 1999), and which has been reported to be a potential source for anticancer, antimicrobial and antiviral medicines (Schempp et al., 2002; Pasqua et al., 2003). More specifically, they grew shoots of the plant for 42 days under well watered and fertilized conditions in a greenhouse, where the air’s CO2 concentration averaged 360 ppm during the photoperiod, and in computer-controlled environment chambers maintained at a mean photoperiod CO2 concentration of 1000 ppm, with all other environmental conditions being comparable between the two treatments.

On the final day of the study, the researchers determined that the net photosynthetic rates of the plants in the CO2-enriched chambers were 124% greater than those of the plants growing in ambient air, and that their dry weights were 107% greater.  In addition, the extra 640 ppm of CO2 in the high-CO2 treatment increased plant concentrations of hypericin and pseudohypericin (two of the major health-promoting substances in the plants) by just over 100%.  Consequently, the 180% increase in the air’s CO2 content more than doubled the dry mass produced by the well-watered and fertilized St. John’s wort plants, while it also more than doubled the concentrations of hypericin and pseudohypericen found in their tissues, which means that the CO2 increase more than quadrupled the total production of these two health-promoting substances.

Mosaleeyanon et al. (2005) also studied St. John’s wort, growing well watered and fertilized seedlings for 45 days in controlled environment chambers at low, medium and high light intensities (100, 300 and 600 µmol m-2 s-1, respectively) at atmospheric CO2 concentrations of 500, 1000 and 1500 ppm.  On day 43, they measured net photosynthetic rates in all treatment combinations at plant growth conditions; and on day 45 the plants were harvested, and hypericin, pseudohypericin and hyperforin (another important health-promoting substance) were extracted from their leaves and quantified.  Under all three light intensities employed in the study, the 1000-ppm increase in atmospheric CO2 concentration experienced in going from 500 to 1500 ppm produced total plant biomass increases of approximately 32%.  Over this same CO2 range, hypericin concentrations rose by 78, 57 and 53%, respectively, under the low, medium and high light intensities, while corresponding increases in pseudohypericin were 70, 57 and 67%, and those in hyperforin were 102, 23 and 3%.  Last of all, compared to results obtained from plants growing out-of-doors in air of 380 ppm CO2 and at light intensities on the order of 1770 µmol m-2 s-1, Mosaleeyanon et al. report that total plant biomass was fully 30 times greater in the high-light, high-CO2 treatment, while concentrations of hypericin and pseudohypericin were 30 and 41 times greater, respectively, in the high-light, high-CO2 treatment.  Consequently, the researchers demonstrated that growing St. John’s wort plants in CO2-enriched air in a controlled environment can enormously enhance the production of both plant biomass and total hypericin and pseudohypericin contents.

Introducing their study on the effects of elevated CO2 on broccoli, Schonhof et al. (2007) note that the glucosinolates found in broccoli comprise a group of bioactive compounds that are responsible for many physiological effects, including serving as feeding deterrents and protective compounds that help protect the plants against herbivore attack (Mewis et al., 2005), enhancing flavor for human consumers, and – perhaps most important of all – helping to prevent cancer in those who consume them (Mikkelsen et al., 2002).

In a set of three experiments conducted in a controlled greenhouse environment, Schonhof et al. grew well watered and fertilized broccoli (Brassica oleracea var. italica Plenck) cv Marathon plants in large soil-filled containers at ambient (430-480 ppm) and elevated (685-820 ppm) atmospheric CO2 concentrations to the stage where fully developed heads could be harvested for glucosinolate analyses.  Results indicated that the roughly 65% increase in atmospheric CO2 concentration increased the fresh weight of the broccoli heads by approximately 7%, while it increased the total glucosinolate concentration of the broccoli inflorescences by 14%, due primarily to identical 37% increases in two methylsulfinylalkyl glucosinolates: glucoiberin and glucoraphanin.  Based on these results Schonhof et al. concluded by saying that atmospheric CO2 enrichment “can enhance the health-promoting quality of broccoli because of induced glucosinolate content changes.”

In a separate study, Ziska et al. (2005) grew well watered and fertilized tobacco and jimson weed plants from seed in controlled environment chambers maintained at atmospheric CO2 concentrations of either 294 ppm (reduced), 378 ppm (ambient) or 690 ppm (elevated) and mean air temperatures of either 22.1 or 27.1°C for 50 and 47 days after planting for tobacco and jimson weed, respectively, while sampling the plants at weekly intervals beginning at 28 and 16 days after planting for tobacco and jimson weed, respectively, to determine the effects of these treatments on the concentrations of three plant alkaloids possessing important pharmacological properties: nicotine, in the case of tobacco, and atropine and scopolamine, in the case of jimson weed.  In following these protocols, they found that at the time of final harvesting, the elevated CO2 had increased the aboveground biomass production of tobacco by approximately 89% at 22.1°C and 53% at 27.1°C, and to have increased that of jimson weed by approximately 23% and 14% at the same respective temperatures.  It was also found to have reduced the concentration of nicotine in tobacco, to have increased the concentration of scopolamine in jimson weed, but to have had no significant effect on the concentration of atropine in jimson weed. These changes (reduced nicotine in tobacco and increased scopolamine in jimson weed) would likely be characterized as positive; for the researchers report that nicotine is acknowledged to have significant negative impacts on human health, and that scopolamine is used as a sedative and as “an antispasmodic in certain disorders characterized by restlessness and agitation, (e.g., delirium tremens, psychosis, mania and Parkinsonism).”

With respect to the significance of their findings, Ziska et al. say “it can be argued that synthetic production of these secondary compounds alleviates any concern regarding environmental impacts on their production from botanical sources; however, developing countries (i.e., ~75% of the world population) continue to rely on ethno-botanical remedies as their primary medicine (e.g. use of alkaloids from jimson weed as treatment for asthma among native Americans and in India),” also noting that “for both developed and developing countries, there are a number of economically important pharmaceuticals derived solely from plants whose economic value is considerable (Raskin et al., 2002).”

Another plant with a long history of medicinal use is the ginseng plant.  Well known for its anti-inflammatory, diuretic and sedative properties, and acknowledged to be an effective healing agent (Gillis, 1997; Ali et al., 2005), ginseng is widely cultivated in China, South Korea and Japan, where it has been used for medicinal purposes since Greek and Roman times.  Normally, four to six years are required for ginseng roots to accumulate the amounts of the various phenolic compounds that are needed to produce their health-promoting effects.  Consequently, in an effort to develop an efficient culture system for the commercial production of ginseng root, Ali et al. (2005) investigated the consequences of growing ginseng roots in suspension culture in bioreactors maintained in equilibrium with air enriched to CO2 concentrations of 10,000 ppm, 25,000 ppm and 50,000 ppm for periods of up to 45 days.

Of most immediate concern in such an experiment are the effects of the ultra-high CO2 concentrations on root growth, whether or not they be toxic and lead to biomass reductions or even root death.  According to the results, the answer was no.  After 45 days of growth at 10,000 ppm CO2, root dry weight was increased by fully 37% relative to the dry weight of roots produced in bioreactors in equilibrium with ambient air, while root dry mass was increased by a lesser 27% after 45 days at 25,000 ppm CO2 and by a still smaller 9% after 45 days at 50,000 ppm CO2.  Consequently, although the optimum CO2 concentration for ginseng root growth likely resides somewhere below 10,000 ppm, the concentration at which root growth is reduced below that characteristic of ambient air resides somewhere significantly above 50,000 ppm, for even at that extremely high CO2 concentration, root growth was still greater than it was in ambient air.

Almost everything else measured by Ali et al. was even more enhanced by the ultra-high CO2 concentrations they employed in their experiment. After 45 days of treatment, total root phenolic concentrations were 58% higher at 10,000 ppm CO2 than at ambient CO2, 153% higher at 25,000 ppm CO2 and 105% higher at 50,000 ppm CO2, as best as can be determined from the bar graphs of their results.  Likewise, total root flavonoid concentrations were enhanced by 228%, 383% and 232%, respectively, at the same ultra-high CO2 concentrations, while total protein contents rose by 14%, 22% and 30%, non-protein thiol contents by 12%, 43% and 62%, and cysteine contents by 27%, 65% and 100% under the identical respective set of conditions. What is more, there were equally large CO2-induced increases in the activities of a large number of phenol biosynthetic enzymes.

With regard to the implications of their results, Ali et al. write that “the consumption of foodstuffs containing antioxidant phytonutrients such as flavonoids, polyphenolics, ascorbate, cysteine and non-protein thiol is advantageous for human health,” citing Cervato et al. (2000) and Noctor and Foyer (1998).  Hence, they conclude that their technique for the culture of ginseng roots in CO2-enriched bioreactors could be used for the large-scale production of an important health-promoting product that could be provided to the public in much greater quantities than is currently possible.

Another plant that serves as both an herbal ingredient and a food delicacy in China, Japan and Korea is the brown seaweed Hizikia fusiforme, which has been studied by Zou (2005), who collected specimens of it from intertidal rocks along the coast of Nanao Island, Shantou, China, and maintained them in glass aquariums in filtered natural seawater enriched with 60 µM NaNO3 and 6.0 µM NaH2PO4, where the plants were continuously aerated with either ambient air of 360 ppm CO2 or CO2-enriched air of 700 ppm CO2.  Under these conditions, Zou measured the seaweed’s relative growth and nitrogen assimilation rates, as well as its nitrate reductase activity.  This work revealed that the slightly less than a doubling of the air’s CO2 concentration increased the seaweed’s mean relative growth rate by about 50%, its mean rate of nitrate uptake during the study’s 12-hour light periods by some 200%, and its nitrate reductase activity by approximately 20% over a wide range of substrate nitrate concentrations.

In discussing the implications of these findings, Zou notes that “the extract of H. fusiforme has an immunomodulating activity on humans and this ability might be used for clinical application to treat several diseases such as tumors (Suetsuna, 1998; Shan et al., 1999).”  He also reports that the alga is “becoming one of the most important species for seaweed mariculture in China, owing to its high commercial value and increasing market demand.”  As a result, the ongoing rise in the air’s CO2 content bodes well for both of these applications.  In addition, Zou says “the intensive cultivation of H. fusiforme would remove nutrients more efficiently with the future elevation of CO2 levels in seawater, which could be a possible solution to the problem of ongoing coastal eutrophication,” which in turn suggests that rising atmospheric CO2 concentrations may also assist in the amelioration of this environmental problem.

Also working with a marine alga – specifically, unicellular Nannochloropsis sp. – was Hoshida et al. (2005), who grew the alga in batch culture under normal (370 ppm) and elevated (3000 and 20,000 ppm) atmospheric CO2 concentrations in an attempt to learn how elevated CO2 impacted the alga’s production of eicosapentaenoic acid (EPA), a major polyunsaturated omega-3 fatty acid that may play an important role in human health, including the prevention of cardiovascular diseases (e.g. atherosclerosis, thrombogenesis) and the inhibition of tumor growth and inflammation (Dyerberg et al., 1978; Hirai et al., 1989; Kinsella et al., 1990; Sanders, 1993). They also note that “Nitsan et al. (1999) showed that supplementing the diet of hens with Nannochloropsis sp. led to an increased content of n-3 fatty acids in the egg yolk, indicating an additional role in enhancing the nutritional value of eggs.”  Likewise, they indicate that “feeding Nannochloropsis sp. to rats caused a significant increase of the content of n-3 polyunsaturated fatty acids (Sukenik et al., 1994),” suggesting it may play an “important role as the source for n-3 polyunsaturated fatty acids in human nutrition.”

What the Japanese scientists learned from their experiment was that “maximum EPA production was obtained when 20,000 ppm CO2 was supplied 12 hours prior to the end of the exponential growth,” and that “the total EPA production during 4-day cultivation was about twice that obtained with ambient air.” They also report that other researchers have obtained similar results, noting that EPA is mainly contained in thylakoid membranes (Sukenik et al., 1989; Hodgson et al., 1991), and that prior experiments have shown that “the amount of stroma thylakoid membrane increased in several plants under elevated CO2 concentrations (Griffin et al., 2001).”  In addition, they say that “in Synechococcus lividus, reduction and synthesis of thylakoid membrane occurred by CO2 deprivation and elevation, respectively (Miller and Holt, 1977),” and that “in Chlorella vulgaris, altering the ambient CO2 concentration varied fatty acid composition (Tsuzuki et al., 1990).”  Last of all, they say that “the effect of CO2 on fatty acid composition and/or fatty acid content was reported in algae and higher plants (Tsuzuki et al., 1990; Sergeenko et al., 2000; He et al., 1996; Radunz et al., 2000),” and that “increased EPA production caused by elevated CO2 concentration was reported in P. tricornutum (Yongmanitchai and Ward, 1991).”  Consequently, as the atmosphere’s CO2 concentration continues to rise, concentrations of omega-3 fatty acids should be widely enhanced in both aquatic and terrestrial plants, thereby benefiting much of the animal life of the planet.

With respect to the major staple crop of soybeans, Caldwell et al. (2005) write that “the beneficial effects of isoflavone-rich foods have been the subject of numerous studies (Birt et al., 2001; Messina, 1999),” and that “foods derived from soybeans are generally considered to provide both specific and general health benefits,” presumably via these substances.  Hence, the authors set out to determine how the isoflavone content of soybean seeds might be affected by the ongoing rise in the air’s CO2 content.

The three researchers grew well watered and fertilized soybean plants from seed to maturity in pots within two controlled-environment chambers, one maintained at an atmospheric CO2 concentration of 400 ppm and one at 700 ppm.  The chambers were initially kept at a constant air temperature of 25°C; but at the onset of seed fill air temperature was reduced to 18°C until seed development was complete, in order to simulate average outdoor temperatures at this stage of plant development.  In a second experiment, this protocol was repeated, except that the temperature during seed fill was maintained at 23°C, with and without drought (a third treatment), while in a third experiment, seed-fill temperature was maintained at 28°C, with or without drought.

In the first experiment, where air temperature during seed fill was 18°C, the elevated CO2 treatment increased the total isoflavone content of the soybean seeds by 8%.  In the second experiment, where air temperature during seed fill was 23°C, the extra CO2 increased total seed isoflavone content by 104%, while in the third experiment, where air temperature during seed fill was 28°C, the CO2-induced isoflavone increase was 101%.  Then, when drought-stress was added as a third environmental variable, the extra CO2 boosted total seed isoflavone content by 186% when seed-fill air temperature was 23°C, while at a seed-fill temperature of 28°C, it increased isoflavone content by 38%.  Under all environmental circumstances studied, therefore, enriching the air with an extra 300 ppm of CO2 increased the total isoflavone content of soybean seeds.  In addition, the percent increases measured under the stress situations were always greater than the percent increase measured under optimal growing conditions.

A second research team to study soybeans within this context and timeframe was that of Kim et al. (2005), who add that important flavoniods “are mainly found in the form of isoflavones in soybean seeds,” including “phytoestrogens with various biological potentials such as antioxidative, pharmaceutical, oestrogenic and anticarcinogenic properties, with some acting as antiestrogens and being used as anticancer agents (Peterson and Barnes, 1991; Anderson et al., 1995; Anthony et al., 1996; Arjmandi et al., 1996; Holt, 1997, Chung et al., 2000).”  In their study, well watered plants were grown from seed to maturity in pots of sandy loam soil within the closed-environment plant growth facility of the National Horticultural Research Institute of Korea, where the plants were exposed to either natural solar radiation and the natural daily course of ambient air temperature or elevated air temperature (= ambient + 5°C) with either no added nitrogen or added nitrogen equivalent to an extra 40 kg N/ha, and where they were maintained at either ambient CO2 (360 ppm) or elevated CO2 (650 ppm).  Then, at the end of the growing season, the plants were harvested and their total biomass determined, while the concentrations of 12 different isoflavones found in their seeds were quantitatively analyzed.  These isoflavones included three aglycons (daidzein, genistein, glycitein), three glucosides (diadzin, genistin, glycitin), three acetyl conjugates (6″-O-acetyldaidzin, 6″-O-acetylgenistin, 6″-O-acetylglycitin), and three malonyl conjugates (6″-O-malonyldaidzin, 6″-O-malonylgenistin and 6″-O-malonylglycitin).

The results of this study indicated that the CO2-induced increase in total plant biomass at normal ambient temperatures was 96% in the case of no added nitrogen and 105% in the case of added nitrogen, while at the warmer temperatures it was 59% in the case of no added nitrogen and 68% in the case of added nitrogen. With respect to seed isoflavone concentrations, the CO2-induced increases of all twelve isoflavones were fairly similar to each other.  As a group, at normal ambient temperatures the mean increase was 72% in the case of no added nitrogen and 59% in the case of added nitrogen, while at the warmer temperatures it was 72% in the case of no added nitrogen and 106% in the case of added nitrogen.  Irrespective of soil nitrogen status and air temperature, therefore, increases in the air’s CO2 content produced large increases in soybean biomass, as well as soybean seed concentrations of twelve major isoflavones.  Hence, as the above studies show, as the atmosphere’s CO2 concentration continues to rise in the years and decades ahead, both the amount and potency of many important health-promoting substances produced in soybean seeds should be significantly enhanced.

Noting that “among medicinal plants, the therapeutic uses of opiate alkaloids from poppy (Papaver spp.) have long been recognized,” Ziska (2008) set out to “evaluate the growth and production of opiates for a broad range of recent and projected atmospheric carbon dioxide concentrations using wild poppy (P. setigerum) as a surrogate for P. somniferum.

Well watered and fertilized plants were grown from seed (one plant per 2.6-liter pot filled with a 4:1:1 mixture of sphagnum, perlite and vermiculite) within growth chambers maintained at four different atmospheric CO2 concentrations – 300, 400, 500 and 600 ppm – for a period of 90 to 100 days, after which the authors quantified plant growth and the production of secondary compounds including the alkaloids morphine, codeine, papaverine and noscapine, which were derived from latex obtained from capsules produced by the plants.  The data indicated that relative to the plants grown at 300 ppm CO2, those grown at 400, 500 and 600 ppm produced approximately 200, 275 and 390% more aboveground biomass, respectively, as best as can be determined from their bar graphs.  In addition, the authors report that “reproductively, increasing CO2 from 300 to 600 ppm increased the number of capsules, capsule weight and latex production by 3.6, 3.0 and 3.7 times, respectively, on a per plant basis,” with the ultimate result that “all alkaloids increased significantly on a per plant basis.”  Based on these results, Ziska et al. concluded that “as atmospheric CO2 continues to increase, significant effects on the production of secondary plant compounds of pharmacological interest (i.e. opiates) could be expected,” which effects, in their words, “are commonly accepted as having both negative (e.g. heroin) and positive (e.g. codeine) interactions with respect to public health.”

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Keys to Optimal Nutrient Levels in Soiless and Wet Hydroponic Grows

By Silas Sativarius

The cannabis industry is without question on the absolute cutting edge of plant nutrient science. While the perfect nutrient combination may have a greater effect on Cannabis yield than other crops, there is no concrete evidence of that. But the real reason nutrient research and products are booming in the Cannabis cultivation industry is simply the vast amount of money Cannabis growers are willing to spend on nutrients compared to ANY other commercial crop. The difference is literally ten fold higher than any other crop bar none.

And with this willingness to spend for the “best” has come a plethora of exaggerated claims to support this huge market. I will not try to evaluate any specific products or claims in this article, but instead I will attempt to help you understand the basics of how to best use your expensive and high tech nutrients.

Nutrient Concentrations and Feeding
Let’s start with the fact that overfeeding or excessive nutrient ppm levels is THE most common mistake inexperienced or ambitious growers make. One misconception that can lead to this mistake is the idea that the maximum level of ppms a plant can handle continues to go up proportionally as the plants growth rate increases. In reality, the maximum PPM level a plant can tolerate, or better, that is optimum (they aren’t the same) is based on a very complex relationship of factors in the root zone far too complicated to discuss here. But the bottom line is, the optimum is relatively static within a narrow range and does NOT increase proportionally with plant growth. So how does the plant get more nutrients as it grows faster? Water transpiration.

Water Transpiration
Water transpiration is the amount of water that literally flows through the plant from the roots to the leaves and out into the atmosphere. There are strict limitations on the concentration of minerals in the water flowing through the Xylem (the plants vascular system) So the more water transpiring, the more nutrients are transported. This is the most important factor in nutrient uptake. And it depends on several factors.

1) Temperature
The higher the temperature, the faster the plant will transpire water to cool the plant. CO2 intake also increases. See 3 Keys for Maximizing.. for more details on Co2 and Temp. Higher temperatures also increase evaporation rates.

2) Humidity
The lower the relative humidity (RH) i.e. the dryer the air, the less resistance to evaporation and the easier it is for the plant to evaporate water from the vacuoles in the leaves. The more water taken IN, the more must go OUT, so decreasing humidity helps facilitate water transpiration and prevents the potential for water condensation on the leaves which further restricts transpiration and makes the plant vulnerable to fungi like mildew.

3) Root capacity
The more root capacity the plant has, which literally comes down to the total surface area of the entire root system, the faster water/nutrients can be absorbed. Root surface area can be dramatically increased in two ways, optimizing air space in the soil, and Mycorrhiza.
• Air space – in general, the optimum ratio of air to water in the root zone is 50/50. Soil porosity or the amount of air space in the root zone throughout the life of the plant is CRITICAL to root function, and lot’s of air space creates “feathery” roots with high surface area.
Mycorrhiza – Mycorrhiza are a variety of fungi that grow on the root surface creating a webbing network that not only dramatically increase the functional surface area of the root, but also helps break down and make more available certain nutrient compounds. More on Mycorrhiza in the upcoming Root Zone article.

4) Osmotic pressure
Osmotic pressure is created by the ratios of solutes (minerals) between the exterior and interior of the root. It is here that nutrient PPM really comes into play because lower PPM’s decrease Osmotic pressure, but too high will stop transport completely. So for this reason, unless you suspect you have excessive nutrient levels in the root zone or salt buildup, plants should never be watered with pure Reverse Osmosis (RO) water, for it can negatively impact transpiration.

So you can see that keeping the root zone conditions optimal is ESSENTIAL to both nutrient and water uptake.

Evaporation
Evaporation in this context is the root zone moisture that is evaporated away from the roots without being used by the plant. When this moisture evaporates, it leaves the dissolved nutrients behind, initially just increasing the nutrient ppm level in the root zone, but ultimately, if the root zone gets too dry, usually towards the top, the nutes will dry completely and oxidize into salts, which are difficult to redissolve and create unhealthy conditions in the root zone.

There are 2 environmental factors that effect evaporation. Air and/or root-zone temperature, and air relative humidity (RH). Both an increase in temp and /or a decrease in RH will increase evaporation. Also, the “airier” or more porous the media and/or container, the faster the evaporation.

To compensate for this loss of unused water in the root zone, nutrient levels have to be reduced to effectively keep the existing root zone nutrient ppms consistent. This can be accomplished by either lowering feed water ppms on-going, or by periodically watering with 1/4 to 1/3 strength normal nutrient mix. The amount of reduction depends on the level of evaporation, but a good way to test this is to water the plants starting with a 10-20% reduction and measure the ppms of the nutrient run off coming out of the media. Adjust the feeding nutrient ppm until you get roughly the same coming out (or within 100ppm or so) as going in, each feeding. And remember. The harder you push the plant, i.e. the closer you run it to that maximum threshold, the less headroom you have for environmental fluctuations.

LED vs HPS/MH
As mentioned in Tips and Tricks for LED’s,  LEDs produce no Infra Red (IR) and consequently require higher ambient air temperatures to achieve similar plant metabolism rates to that achieved with HPS, all other factors being equal. With this increase in air temp comes an increase in evaporation, so it is extra important when using LED’s to adjust your ppm schedule to accommodate these changes.

Chelation
There is another factor that has a big impact on both the ability of the plant to absorb nutrients, and consequently your optimum levels of nutrient ppm. It is Chelation.

Chelates are compounds that react with the more stable nutrient molecules found in fertilizers (so they can stay unchanged in the package) and make them less stable, but far more absorbable by the plant. Chelates can also break down harmful salts, turning them back into absorbable nutrients. EDTA, Humic Acid, and Fulvic acid are the most common Chelates used in fertilizers. EDTA and other Chelates are also the active ingredient in most “Final Flush” type additives, (such as Advance’s Final Phase) advertised for use in the last two weeks of flower because they break down salt residue preventing nute lock up in the crucial final weeks.

Chelates are maybe the one nutrient ingredient that really can have a significant impact on growth rate because they make the nutrients more easily absorbed. There really isn’t any toxic threshold for these compounds, but the more you use of them, the less actual nutrients you can add to maintain a safe PPM level, so it is a balancing act.

TIP FROM SILAS – I personally use Liquid Karma (and no they don’t pay me to say this, I just love the stuff) as my organic Humic / Folic acid chelation agent (as well as providing some vitamins) through all phases of growth and I have found that while I may adjust the ratios of the various nutrients –nitrogen, potassium, phosphorus etc—for the different stages, i.e. veg, pre-flower, flower, and ripening, I slowly increase the ratio of Karma to Nutrients from 1 tsp/ gal Karma in Veg, to as high as 4tsp/gallon Karma at peak flower. Do it and WATCH what happens! I will give more details of this and all topics discussed on this site in my “Secrets to Massive Cannabis Yields” book coming out soon. There are many other sources besides Karma for organic Humic /Folic acid chelation additives so take your pick, but Karma is the only nute I have used religiously for almost a decade. I also add a little EDTA additive (1/4 label) anytime I use chemical nutes or flower boosters.

Because chelates make the dissolved nutrients (and salts) suddenly more absorbable to the plant, they can take a perfectly safe ppm level and make it toxic. Thus, whenever you use Chelation additives, you should reduce the nutrient ppm levels by 20% or so and then increase them slowly over time. And never use an EDTA flushing agent at full strength immediately after feeding with nutrients at full strength. Always flush with clear water at least once prior to using these additives at label strength. However, they are actually more effective if used throughout the grow cycle at roughly ¼ label strength, but nutrient PPMS must be adjusted accordingly. As with any changes in nutrient PPM, start low, say 6-700ppm and gradually increase and watch carefully. The fastest way to detect nutrient toxicity is a rapid browning of the normally white stamens of the flower, (Literally over-night) anytime before the last week of flower. This will happen before the leaves may show any symptoms. Spraying of flowers can have the same effect however. Also, browning of the leaf tips can be a sign of longer term excessive nutrient levels.

Root cleaning
As roots grow, they slough off their outer skin like a snake. This skin, is a carbohydrate material and can accumulate and reduce the water /nutrient uptake of the root. In organic humus based soil, there are naturally occurring bacteria such as Bacillus Subtilis and Bacillus Cereus, that eat (breakdown) the organic material in the soil as well as the carbohydrates shed by the roots. These are the same bacteria that break down plant matter in a compost pile.

However, in wet or soiless hydro, healthy root bacteria (flora) do not occur naturally. You have to add the bacteria and then provide them food ongoing, because most hydroponic nutrients do not have significant organic components that could provide food for these organisms, and they will quickly reproduce beyond the amount of food the roots are producing and then begin to die off. And without them, the root system will not be able to uptake water or nutrients efficiently, or breakdown any extra organic material from organic based nutrients that can accumulate and dense up soiless medias, reducing the oxygen present.

So it is important for proper nutrient uptake, that from the first week in veg, you supply periodic doses of healthy bacteria through supplements or compost teas to keep the roots and media clean and healthy, and it is strongly advised that you include a small amount of simple sugar (Glucose or Fructose) to feed the existing bacteria when the root detritus runs low (i.e your roots are clean). ¼ the label recommendation of most sugar products is plenty, but don’t worry, if the sugars are simple forms like glucose or fructose, they can be absorbed by the roots (and leaves) and thus can replace some of the sugars the plant normally has to produce through photosynthesis. Feeding sugar will also replace a small part of the nitrogen requirements. But DO NOT use excessive sugars in the nutrients because it can cause unhealthy conditions in the root zone. Less is more, and 50 (normal) to 100ppm (late flower) is a safe upper limit for sugar supplementation. People go WAY overboard with sugar, let me be clear, sugar DOES NOT translate directly into fragrance or resin. Phosphorus drives resin production. I will discuss this more in future articles.

ph_nutrient_availability_bal420

PH
Normally when plants grow in organic soil with all that good humus being acted on by countless bacteria and fungi, the soil will naturally stabilize around a PH of 6.0-6.5. This is adequate for proper absorption of organic forms of nutrients which are more stable and slightly less bio-available, and watering with simple PH7 water like rain does not alter the PH of this system virtually at all.

But when you are growing in a Hydroponic environment, both wet, aeroponic, or soil-less (peat, rockwool, etc..) the media the plant is growing in has no organic material except what you add in the nutrients. For this reason, it is very difficult to establish prolific enough flora (bacteria etc..) to really buffer the PH conditions. Thus YOU have to adjust your PH on every feeding to the correct level.

Different minerals (nutrients) favor different PH ranges, but when you distill it all down with the dozen or so minerals really essential to an optimally healthy plant, the sweet spot for all hydroponics (not Soil) turns out to be PH 5.7- 6.0. Phosphorus is the most sensitive nute to PH above 6.0 and it is ESSENTIAL to high quality Cannabis. And if you want optimum growth, and you are not using exclusively organic nutrients, you had better keep your PH between 5.7 and 6.0 ALL THE TIME. I’m not going to try to explain it here, but look at the chart above and suffice to say, you need to keep your PH in that range EVERY TIME you feed. Not just when you mix the tank. Every time you feed, RECHECK IT. Be diligent, and you will notice the difference.

And buy a GOOD PH meter and keep a back up meter as a reference, because when they fail, they don’t tell you. They just give you incorrect readings and you won’t know till it’s too late and your wondering why everything is SH*T. And it can only take a couple feedings that are PH 6.2 or higher, especially during the CRUCIAL first 2-3 weeks of flower, and especially if your using chemical nutes, to ruin an entire crop from Phosphorus deficiency. In those first weeks, the plants aren’t sucking up the nutes as fast, so it sits there longer, and often there is a bunch of fresh clean media (Peat, etc..) from a recent re-pot to a larger media, so there’s just not much resident organic residue and bacteria to help buffer the PH, so it has lots of time to mess with your plants. And it will RUIN an entire crop. Oh the plants will still grow, but they will never be optimal, and there is no fixing it.

You can protect yourself from PH issues somewhat by always using an organic Base nutrient. Also, bacterial treatments help as well. Organic nutes are less PH sensitive and over time leave behind material that will set this natural PH buffer mentioned previously. But this material also slowly clogs up the media, so if you use organics with soil-less media, increase the aeration in your starting media with additional perlite etc.. But ask any pro grower, organics nutes are safer and easier, and make for a little better taste and smell etc.. but plants grow much faster and bigger with high quality chelated chemical nutes. So it is a tradeoff. For Wet Hydro, organics will simply clog your jets and kill things, so you have to be very careful in avoiding nutes with organic particulates, which is in almost all of them. I will get into more detail in future publications.

But spend the money, spend the time, and watch your PH like a HAWK. 5.8-6.0. 6.0 for the first 2-3 weeks to favor Nitrogen and keep them good and green, then you can slowly begin to slide it down towards 5.8 during ripening to favor Phosphorus and Potassium absorption for flower production. Doing so will make the sun leaves begin to yellow, but don’t worry, that’s ok, they are less important at this stage than high phosphorus absorption.

TIP FROM SILAS:  Ammonium based nutrients will slowly decrease the media PH,  most other forms of nutrients will slowly increase media PH.  So ammonium based nutes will be less sensitive to excess (>6.0) PH levels because the absorption at the root naturally creates free hydrogen that drops the PH. If your media or soil PH is too low (exp. general leaf yellowing from nitrogen starvation), use non-ammonium based nutrients to correct.

Conclusion
I hope I have not sacrificed too much literal accuracy (my disclaimer for all you PHD plant biologists out there) in the interest of simplification here, but I think you can see that optimizing nutrient uptake is a very complex system that requires careful and gradual adjustments to find the optimal levels for your plant and environment. And the most important thing to remember is — of the core factors in plant metabolism (growth rate), namely Light, CO2, Temperature, Water, and Nutrients — Nutrients are the LEAST important factor in optimizing yield, and the most problematic. Ppm levels DO NOT need to be raised significantly as plant metabolism goes up, because the plant naturally increases the amount of water and nutrients taken up into the plant proportionally with the metabolism. You are better served by optimizing the other elements that facilitate water transpiration, namely temp, humidity, and root health. Increasing nutrients should be the last consideration in trying to optimize yield, and you should always be conservative. There is little downside to running conservative PPM’s and LOTS of potential downside to running too much.

To those who grow, we salute you!